hs 5  (ATCC)

Journal: NPJ Precision Oncology

Article Title: Venetoclax resistance in preclinical KMT2A-rearranged acute lymphoblastic leukemia models is characterized by high inter- and intra-model heterogeneity

doi: 10.1038/s41698-025-01249-1

Figure Lengend Snippet: A Tumor cell proliferation was monitored by longitudinal full body bioluminescence imaging. Representative images of three animals per group. Mouse IDs are given for each animal to allow for allocation of individual animals in ( B , C ). B Bioluminescence signals from dorsal and ventral images were quantified. 5–8 animals per group, each line represents an individual animal. Mice displayed in ( A ) are marked with the respective mouse ID. Imaging was discontinued once technical signal saturation was achieved. Kolmogorov–Smirnov test for each time point. * p < 0.05. C Quantification of GFP + tumor cell frequency in peripheral blood using flow cytometry. 5–8 animals per group, each line represents an individual animal. Mice displayed in ( A ) are marked with the respective mouse ID. Kolmogorov–Smirnov test for each time point. * p < 0.05; ** p < 0.01. D Kaplan–Meier survival analysis. 6–8 animals per group, Log-rank test. ** p < 0.01. E Relative tumor cell doubling times were calculated based on bioluminescence data ( B ; early proliferation) and peripheral blood flow cytometry values ( C ; late proliferation) and compared to time-matched vehicle cohorts. Mean ± SD of 5–8 animals per group, multiple data sets per animal during the exponential growth phase. Kolmogorov–Smirnov test. * p < 0.05; ** p < 0.01; *** p < 0.001. F Determination of blast frequency in blood, bone marrow and spleen by flow cytometry when the mice reached humane endpoints (30% blasts in blood or weak performance status). Mean ± SD of 4-6 animals per group. Welch’s t test. G Isolated VEN-resistant bone marrow cells were spun onto microscopic slides and Pappenheim stained. Representative images of 5–8 mice per group, ×100 magnification.

Article Snippet: SEM or RS4;11 cells were cultivated alone or cocultured with human stromal bone marrow cells (HS-5 cell line, ATCC, Manassas, VI, USA) and incubated with DMSO (control) or VEN for 72 h. Absolute cell counts were assessed by trypan blue staining and subsequent microscopic quantification.

Techniques: Imaging, Flow Cytometry, Isolation, Staining

Journal: NPJ Precision Oncology

Article Title: Venetoclax resistance in preclinical KMT2A-rearranged acute lymphoblastic leukemia models is characterized by high inter- and intra-model heterogeneity

doi: 10.1038/s41698-025-01249-1

Figure Lengend Snippet: A Quantification of CD45 + /CD19 + tumor cell frequency in peripheral blood using flow cytometry. 1–3 animals per group, each line represents an individual animal. B Kaplan–Meier survival analysis. All five PDX models were summarized. 15 animals per group, Log-rank test. * p < 0.05. C Tumor cell doubling times were calculated based on peripheral blood flow cytometry values ( A ). Mean ± SD of 1–3 animals per group, multiple data sets per animal during exponential growth phase. Unpaired t test. D Tumor cell doubling times summarized for all five PDX models. Mean ± SD of 1–3 animals per group, multiple data sets per animal during exponential growth phase. Kolmogorov–Smirnov test. E Determination of blast frequency in blood, bone marrow and spleen by flow cytometry when the mice reached humane endpoints (30% blasts in blood or weak performance status) or study endpoint (120 days post tumor cell injection). Mean ± SD of 1–3 animals per group. Kolmogorov–Smirnov test. F Tumor cell frequency in blood, bone marrow and spleen at experiment termination, summary of all five PDX models. Mean ± SD of 1–3 animals per group. No statistical assessment due to animals lacking VEN resistance. G Isolated VEN-resistant bone marrow cells were spun onto microscopic slides and Pappenheim stained. Representative images of 1–3 mice per group, 100x magnification. No statistic assessment of data from patients #0054 and #0152 derived models due to animals VEN-treated animals lacking resistance establishment.

Article Snippet: SEM or RS4;11 cells were cultivated alone or cocultured with human stromal bone marrow cells (HS-5 cell line, ATCC, Manassas, VI, USA) and incubated with DMSO (control) or VEN for 72 h. Absolute cell counts were assessed by trypan blue staining and subsequent microscopic quantification.

Techniques: Flow Cytometry, Injection, Isolation, Staining, Derivative Assay